Nakong ea secretion, protheine e khomarela BNP (77th ho 108th amino acids) le n-terminal fragment NT-proBNP (1st ho isa ho 76th amino acid).Ha BNP, e bolelele ba li-amino acid tse 32, e kenngoa maling, e tlama ho li-receptor tsa eona (NPRA le NPRB) mme e laola khatello ea mali ka mekhoa e sa tšoaneng.NT-proBNP, e leng 76 amino acid e telele, ha e na ts'ebetso ea likokoana-hloko, empa hobane e na le halofo ea bophelo bo bolelele ho feta BNP, e loketse haholoanyane e le pontšo ea ho lemoha mafu a sa tšoaneng a pelo.Litekong tsa bongaka tsa liphoofolo tse ruuoang lapeng, mali a NT-proBNP ho lintja a phahame ho feta 900 pmol / L, 'me likatse li phahametse 270 pmol / L,' me ho na le kotsi e kholo ea mafu a amanang le pelo.Leha ho le joalo, hoa lokela ho hlokomeloa hore hobane NT-proBNP e ntšitsoe ke liphio, ha phoofolo e tšoeroe ke mafu a amanang le liphio, mahloriso a NT-proBNP 'meleng a tla boela a eketsehe' me a be le maikutlo a fosahetseng a tla hlaha tekong.
Sehlahisoa sena se sebelisa fluorescence immunochromatography ho lemoha litaba tsa fNT-proBNP ho serum/plasma ka bongata.Molao-motheo oa motheo: Ho na le mela ea T le C holim'a lera la faeba ea asiti ea nitric ka ho latellana, 'me mohala oa T o koahetsoe ka antibody a e hlokomelang fNT-proBNP ka ho khetheha.Motsoako oa pad o fafatsoa ka fluorescent e 'ngoe ea nanomaterial e ngotsoeng antibody b e ka khethollang FDT-probNP ka ho hlaka.Mohlala, FDT-probNP e qala ka ho kopanya le nanomaterial e ngotsoeng antibody b ho etsa motsoako, ebe ho ea holimo chromatography, e rarahaneng e kopana le T-line antibody A ho etsa sebopeho sa sandwich.Ha thabo ea mabone a khanya, nanomaterial e hlahisa matšoao a fluorescence.Matla a lets'oao a amana hantle le bongata ba fNT-proBNP sampole.
Ho tloha ha e theoa, feme ea rona e ntse e nts'etsapele lihlahisoa tsa maemo a holimo lefatšeng ka ho latela molao-motheo
ea boleng pele.Lihlahisoa tsa rona li fumane botumo bo botle indastering le ts'epo ea bohlokoa har'a bareki ba bacha le ba khale..