Canine Cortisol (cCortisol) ke hormone ea steroid e hlahisoang ke canine adrenal cortex.Cortisol e thusa ho boloka boemo ba tsoekere maling bo sa feleng, e fokotsa ho ruruha le ho thusa 'mele ho laola khatello ea maikutlo.Le hoja maemo a fapa-fapaneng a bakoang ke litekanyetso tse phahameng ka tsela e sa tloaelehang tsa homone ea tahi a bitsoa Cushing syndrome (CS), lintja le likatse li ka tšoaroa ke CS, e atileng haholo lintjang ho feta likatse.Lintja tsa botsofali le botsofali (tse ka bang lilemo tse 7 ho isa ho tse 12)
ba na le monyetla oa ho ba le lefu lena.Lefu lena le hlaha butle mme matšoao a pele ha ho bonolo ho lemoha.CS e ka fumanoa ka bongaka ka tlhahlobo ea adrenocorticotropic hormone (ACTH) le teko ea khatello ea dexamethasone, le mefuta ea eona e fapaneng: adrenal-dependent (ATH) le pituitary-dependent (PDH).
Sehlahisoa sena se sebelisa fluorescence immunochromatography ho bona ka bongata litaba tsa cCortisol ho serum ea ntja/plasma.Molao-motheo oa motheo: Methapo ea T le C e tšoailoe lera la nitrocellulose, mohala oa T o tlositsoe ka cCortisol antigen a, 'me pate e tlamang e fafatsoa ka fluorescent nanomaterial e ngotsoeng antibody b e ka lemohang cCortisol ka ho khetheha.
cCortisol ea sampole e qala ho ngoloa ka nanomaterial.Lesole la 'mele la b le tlama ho etsa ntho e rarahaneng, ebe chromatographs e ea holimo.E rarahaneng e qothisana lehlokoa le T-line antigen a 'me e ke ke ea tšoaroa;Ho fapana le hoo, ha ho se na cCortisol sampoleng, antibody b e tlama ho antigen a.Ha lebone la ho hlasimolla le khantšitsoe, thepa ea nano e ntša lets'oao la fluorescent, 'me matla a lets'oao a bapana le kholiso ea cCortisol sampole.
Ho tloha ha e theoa, feme ea rona e ntse e nts'etsapele lihlahisoa tsa maemo a holimo lefatšeng ka ho latela molao-motheo
ea boleng pele.Lihlahisoa tsa rona li fumane botumo bo botle indastering le ts'epo ea bohlokoa har'a bareki ba bacha le ba khale..